Description
Amyl Bacteriological Lactose, RM 297, is suitable for use in bacteriological culture media as a source of carbon and for the differentiation of bacterial genera and species according to their ability to hydrolyse lactose into it’s component molecules, glucose and galactose.
For testing of fermentation reactions, lactose should be added to a carbohydrate free medium, containing an acid-base indicator such as phenol red or bromocresol purple, to a concentration of between 0.5 and 1% (w/v) and sterilised by filtration.
Inoculation of a suitable volume of the sterile, lactose containing medium, followed by incubation under controlled conditions for a suitable period of time, dependant upon the growth requirements of the organism under test, will provide a definitive answer to the organisms ability to ferment lactose, thereby providing one of the criteria required for establishment of the organisms identification.
Quality Control: (as a 0.5% sterile solution incorporated into Carbohydrate Utilisation Broth (Amyl AM 723)
