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LB BROTH

Amyl LB Broth, AM 755 is compounded according to the formulation of a number of workers who developed the medium as a means for the propagation of strains of Escherichia coli used in the study of microbial genetic markers1,2,3,. The medium is used as a growth and maintenance medium for E.coli and other members of the Enterobacteriaceae by the American Type Culture Collection

$32.00 — $905.00

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Amyl LB Broth, AM 755 is compounded according to the formulation of a number of workers who developed the medium as a means for theĀ  propagation of strains of Escherichia coli used in the study of microbial genetic markers1,2,3,. The medium is used as a growth and maintenance medium for E.coli and other members of the Enterobacteriaceae by the American Type Culture Collection4.

The composition of the medium provides for the rapid and luxuriant growth of E.coli, strains of which metabolise the casein peptone (Tryptone) as a source of nitrogen and carbon, while essential growth factors such as co-enzymes, vitamins and minerals are available in the yeast extract. Sodium chloride maintains the osmotic balance of the medium while the low levels of carbohydrate present avoid the need of a buffering system in order to prevent excessive pH changes. The addition of Bacteriological Agar, Amyl RM 250, at a level of 12 grams /litre, provides a solid medium for the propagation of Enterobacteriaceae.


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