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LISTERIA OXFORD SUPPLEMENT

A selective supplement which, when incorporated into Amyl Listeria Oxford Agar Base, (AM 86), is used for the isolation and provisional identification of Listeria monocytogenes from food and dairy products. Conforms to Australian Standard Methods.

$144.40

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The incorporation of Amyl Listeria Oxford Supplement, SP 454, into Amyl Listeria Oxford Agar Base, AM 86, complies with the formulation developed by Curtis et al1 and the requirements specified by the Australian Standard Methods for Food Microbiology for the isolation and provisional identification of Listeria monocytogenes from food and dairy products2,3.

Amyl Listeria Oxford Supplement, SP 454, contains the following selective ingredients: Cycloheximide as an inhibitor of yeast and mould growth, Colistin Sulphate to suppress the growth of Pseudomonas sp., the third generation cephalosporin, Cefotetan, an inhibitor of gram negative bacteria other than Pseudomonads and Fosfomycin, a broad spectrum antibiotic, which acts synergistically to amplify the activity of other antimicrobialsin addition to being active against many gram positive organisms other than Listeria sp. and enterococci.

The selective substances present in Amyl Listeria Oxford Supplement, SP 454, are complementary to the inhibitors already incorporated into the Amyl Listeria Selective Agar Base (Oxford). The level of Acriflavine in the basal medium (5mg/l) has been shown to be less detrimental to the recovery of damaged cells of Listeria, whilst still having an inhibitory effect on many gram positive organisms other than enterococci4. Because strains of Listeria monocytogenes are consistently aesculinase positive, this marker is frequently used to detect and provisionally identify the presence of the organism. However, since enterococci, which may also be present in food and dairy procucts, also possess the same marker, measures must be taken to inhibit their growth on media using aesculinase detection as a preliminary identifier for Listeria monocytogenes, accomplished in Listeria Oxford Agar by the inclusion of Lithium chloride in the medium.

Supplied in box of 10 x 100gm vials.


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