Description
Amyl Novobiocin Lyophilised Supplement, SP 451 is supplied as an ampoule containing 10mgm of lyophilised Novobiocin (Sodium salt) for addition to 500 ml of enrichment broth used for the isolation of strains of enterohaemorrhagic Escherichia coli, (EHEC), O157:H7. The content of each lyophilized ampoule is reconstituted with 10ml of sterile water, mixed thoroughly to dissolve, and added to one of either, Amyl Modified EC Broth, AM 744, Amyl Modified EC Broth No Bile, AM 744-NB or Amyl E.coli Broth, AM 759, following sterilisation of the liquid medium by autoclaving then cooling to 40°C., to give a final level of Novobiocin in the medium of 20mgm/litre.
Supplied in box of 10 x 500ml bottles.