Description
R3A medium was developed from the work of Reasoner & Geldreich1 essentially as a suitable sub-culture medium for organisms which either failed to grow after primary culture on PCA or grow poorly.
Once growth has been established on primary media, bacteria utilise the additional level of nutrients in R3A producing colonies more suitable to undergo biochemical identification, particularly of non coliform organisms.
R3A medium is also used as a primary isolation & sub-culture medium for aquatic organisms.
